Allowing the simultaneous determination of the percentage and the absolute number of cells per microliter of the sample in a single tube.
Identifying non-viable cells and non-specific background signal.
Quick and easy identification of double positive CD45 and CD34.
Isotype control (IgG1) included: highly reliable diagnostic results.
This Kit allows the identification of CD34 + cells and link together their number with the identified known number of spheres discriminating between viable and non-viable cells and discarding false positives produced by non-specific staining of the isotype.
Stem Cell Kit is a kit designed for quantitative determination of hematopoietic stem cells (HSCs) CD34 positive due to the specificity of the following antibodies:
The anti-CD45 antibody recognizes type C tyrosine receptor protein phosphatase, a protein of 220 kD member of Leukocyte Antigen family.
The anti-CD34 antibody recognizes the progenitor hematopoietic cell protein antigen CD34.
Additionally, the number of false positives caused by nonspecific staining can be detected with the use of CD34 isotype control (IgG1)
Stage 1: selection of microspheres population
Stage 2: selection of viable cells population ( section B)
Stage 3: using population from section B (viable cells), identify CD34+ cells
Stage 4: Note the number and the percentage of cells CD34+
25 test CD45/CD34, 25 test CD45/igG1, 50 test stepcount, 50 test 7-AAD, 5ml 10X NH4CI Lysing Solution | Kit content (TDS)
50 test CD45/CD34, 50 test CD45/igG1, 100 test stepcount, 100 test 7-AAD, 10ml 10X NH4CI Lysing Solution | Kit content (TDS)
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