The kit contains an R-phycoerythrin (R-PE) conjugated monoclonal antibody, which recognizes a platelet specific antigen.
The kit also contains polyclonal antibodies conjugated whit fluorescein isothiocyanate (FITC) against total human immunoglobulins, human IgA, IgM and IgG
The kit contains a polyclonal antibody conjugated whit FITC as a negative control.
Immune thrombocytopenic purpura (ITP) is an autoimmune disorder characterised by a low platelet count and mucocutaneous bleeding. The autoantibodies are directed primarily to the platelet-specific receptors CD41a (GPIIb/IIIa) and CD42b (GPIb).
As a result, the sensitized platelets are rapidly cleared by the monocyte-macrophage cell systems. The determination of autoantibodies against thrombocytes allows differentiate immune from nonimmune thrombocytopenia.
Anti-human Igs specificity analysis shows a very low cross-reactivity (>7%) for all of them, making easy the correct identification of non-pathological samples.